Concentrations of the endocannabinoid N-arachidonoylethanolamine in the follicular fluid of women with endometriosis: the role of M1 polarised macrophages

Fonseca et al. ·Reproduction, Fertility and Development Impact 0.4

Unklar 11 Zitate

StichprobeSonstige Evidenz

Dauerunklar

EndpunktAEA-Konzentration

Verblindungn.a.

DesignSonstiges

Kernaussage

Studie zeigt erhöhte AEA-Konzentrationen in Follikelflüssigkeit von Frauen mit Endometriose und dass M1-polarisierte Makrophagen die AEA-Produktion durch Granulosazellen erhöhen; therapeutische oder klinische Effekte werden nicht untersucht.

Zusammenfassung

Messung von Anandamid (AEA) im Follikelfluid bei Frauen mit/ohne Endometriose; explorativ, mechanistische Daten zu endogenem Endocannabinoid-Status, keine Intervention mit Cannabis.

PopulationFrauen mit assistierter Reproduktionstechnologie (ART), darunter Endometriose, männliche Infertilität, tubare/hormonelle/ungeklärte Infertilität; zusätzlich in-vitro-Kokultur humaner Granulosazellen mit Makrophagen

InterventionMessung von N-Arachidonoylethanolamin (Anandamid, AEA) im Follikelfluid; in vitro Kokultur mit M1-polarisierten Makrophagen

OutcomeFF-AEA-Konzentrationen signifikant höher bei Endometriose (2,5 nM) vs. männliche Infertilität (1,6 nM); M1-Makrophagen steigern AEA-Produktion durch Granulosazellen via NAPE-PLD-Hochregulation

Qualitätsprofil

Größe —

Verblindung —

Effektstärke —

Zitate / Jahr ★★★★★

Autoren

Fonseca B M, Moreira-Pinto B, Costa L et al.

DOI 10.1071/rd20247↗

Design: Sonstiges

Abstract

Concentrations of the endocannabinoid N-arachidonoylethanolamine in the follicular fluid of women with endometriosis: the role of M1 polarised macrophages. Although N-arachidonoylethanolamine (AEA; also known as anandamide) is present in human follicular fluid (FF), its regulation remains unknown. Therefore, the aims of the present study were to: (1) investigate the relationships between FF AEA concentrations in women undergoing assisted reproductive technology and their age, body mass index, ART characteristics and fertility treatment outcomes; and (2) assess how different inflammatory patterns may trigger AEA production by human granulosa cells (hGCs). FF AEA concentrations were higher in women undergoing IVF than in those undergoing intracytoplasmic sperm injection group. FF AEA median concentrations were lower in women undergoing ART because of male factor infertility than in women with endometriosis (1.6 vs 2.5nM respectively), but not women with tubal, hormonal or unexplained infertility (1.6, 2.4 and 1.9nM respectively). To evaluate the effects of macrophages on AEA production by hGCs, hGCs were cocultured with monocyte-derived macrophages. The conditioned medium from M1 polarised macrophages increased AEA production by hGCs. This was accompanied by an increase in AEA-metabolising enzymes, particularly N-acyl phosphatidylethanolamine-specific phospholipase D. The results of the present study show that high FF AEA concentrations in patients with endometriosis may be associated with the recruitment of inflammatory chemokines within the ovary, which together may contribute to the decreased reproductive potential of women with endometriosis. Collectively, these findings add a new player to the hormone and cytokine networks that regulate fertility in women.

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